Expression of the Metalloproteinase ADAM8 Is Upregulated in Liver Inflammation Models and Enhances Cytokine Release In Vitro

Figure 1

Upregulation of ADAM8 mRNA expression in different murine models of liver injury. (a–e) Liver tissue was obtained from control mice (Ctrl) or mice with different types of liver injury induced by either treatment with LPS/GalN for 6 h, by bile duct ligation (BDL) (3 weeks), or by partial hepatectomy (PH) (48 h). The mRNA expression of Adam8 (a), TNF-α (b), IL-6 (c), Adam10 (d), and Adam17 (e) in these livers was determined by qPCR. The mRNA expression was normalised to the average of two reference genes: Rps29 and Gapdh. Data are shown as the . Six mice were used in each group. Differences to the control are indicated as asterisks (, , and ). (f) Immunohistochemistry analysis of liver sections from mice treated with or without LPS for 6 h with a polyclonal antibody against ADAM8. Representative tissue stainings for two different mice per group () are shown.

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