Expression of the Metalloproteinase ADAM8 Is Upregulated in Liver Inflammation Models and Enhances Cytokine Release In Vitro

Figure 2

Upregulation of ADAM8 expression in hepatocyte and endothelial cells by LPS. (a–p) Human HepG2 and murine Hepa1-6 hepatoma cells as well as human EA.hy926 and murine LSEC endothelial cells were stimulated with or without LPS (1 μg/ml). After 24 h of stimulation, cells were analysed for mRNA expression of TNF-α (a–d), IL-6 (e–h), and ADAM8 (i–l). The mRNA expression level was determined by qPCR and normalised to the mRNA expression of GAPDH (for human cells) and Rps29 (for murine cells). Additionally, ADAM8 protein expression was detected by Western blot analysis (m–p). The antibody detects a band of approximately 90 kDa, i.e., mature ADAM8. The data are shown as representative blot for each cell line. The band intensities were quantified by densitometry and normalised to GAPDH protein expression. Quantitative data are shown as the of 3-4 independent experiments. Significant differences to the unstimulated controls are indicated by asterisks (, , and ).