Expression of the Metalloproteinase ADAM8 Is Upregulated in Liver Inflammation Models and Enhances Cytokine Release In Vitro

Figure 6

ADAM8 upregulation in cultured liver cells by mediators of steatohepatitis. (a–d) Murine Hepa1-6 and human HepG2 hepatoma cells were either left unstimulated (US) or stimulated with fatty acid (FA), IL-1β, or a combination of both. After 24 h, the cells were analysed for ADAM8 mRNA expression(a, b) and protein expression (c, d). (e–h) Murine LSEC and human EA.hy926 endothelial cells were left unstimulated (US) or stimulated with TNF-α, IFN-γ, or a combination of both (TI). After 24 h, the cells were analysed for ADAM8 mRNA (e, f) and protein expression (g, h). (i–l) Murine GRX and human LX-2 stellate cells were left unstimulated (US) or stimulated with TGF-β. After 24 h, the cells were analysed for ADAM8 mRNA (i, j) and protein expression (k, l). The mRNA expression was analysed by qPCR and normalised to Rps29 for murine cells and to GAPDH for human cell lines. The protein expression was analysed by Western blot, and representative blots are shown for each cell line. The protein expression was quantified by densitometry and normalised to GAPDH. Quantitative data are shown as the of 3-4 independent experiments. Significant differences to the unstimulated controls are indicated by asterisks (, , and ).