Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South China

Figure 2

Identification of the four virus strains by PCR reaction and IFA response. In panel (a) are the PCR products that were electrophoresed on an agarose gel, and the four viruses at 545 bp, showing the specific bands. (b) Agarose electrophoresis results of the full-length ALV-J genome amplified from the four strains of viruses. (c) Uninfected DF-1 cells as a negative control, at 100×. (d–g) GD19GZ01 through GD19GZ04 strains inoculated into the DF-1 cells, respectively; the IFA response indicating specific green fluorescence, at 100×. The numbers in the upper column of the gel chart are for the following: M is the marker, A to D are the ALV-A- to ALV-D-specific primers, J is the ALV-J specific primers for H5/H7, and J1 to J4 represent the four segments of the full length of the ALV-J genome. bp: base pairs. The numbers on the left indicate the lengths of the molecular weight standards.

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