Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South China

Table 1

The primers used to detect the ALVs and to amplify the full-length proviral genome of ALV-J isolates.

Primer^a	Sequence (5–3)	Annealing temperature (°C)	Size of PCR products (bp)
MDV-F	GGATGAGGTGACTAAGAAAG	59	560
MDV-R	CGAACCAAAGGTAACACACG
REV-F	GCCTTAGCCGCCATTGTA	53	383
REV-R	CCAGCCTACACCACGAACA
ALV-A-F	GGATGAGGTGACTAAGAAAG	50	692
ALV-A-R	AGAGAAAGAGGGGTGTCTAAGGAG
ALV-B-F	GGATGAGGTGACTAAGAAAG	50	847
ALV-B-R	TGGACCAATTCTGACTCATT
ALV-C-F	GGATGAGGTGACTAAGAAAG	50	860
ALV-C-F	GAGGCCAGTACCTCCCACG
ALV-D-F	GGATGAGGTGACTAAGAAAG	50	797
ALV-D-F	ATCCATACGCACCACAGTATTCG
ALV-J-H5	GGATGAGGTGACTAAGAAAG	55	545
ALV-J-H7	CGAACCAAAGGTAACACACG
F: J-1F	TGTAGTGTTATGCAATACTCTT	52	2100
F: J-1R	CGACCCAGTTTGTCCATCCCTC
F: J-2F	AGCACCCTCCACGCTGACCAC	59	2100
F: J-2R	GGTGGTCGGTAACCCTCACTTTCA
F: J-3F	GACCCTGCCCTGCCTCTGGAA	59	1800
F: J-3R	CGACGGAAATAATAACCACGCACA
F: J-4F	GCGTGGTTATTATTTCCGTCGTCC	59	2400
F: J-4R	TGAAGCCTTCCGCTTCATGCAGGT