Saussureae Radix Attenuates Neuroinflammation in LPS-Stimulated Mouse BV2 Microglia via HO-1/Nrf-2 Induction and Inflammatory Pathway Inhibition

Table 2

Primary and secondary antibodies used for Western blotting analysis.

Antibody	Corporation	Product no.	RRID	Dilution rate
iNOS	Cell Signaling	#13120	AB_2687529	1 : 1000
COX-2	Cell Signaling	#4842	AB_2085144	1 : 1000
β-Actin	Santa Cruz	#SC-47778	AB_626632	1 : 1000
HO-1	Cell Signaling	#82206	AB_2799989	1 : 1000
Nrf-2	Novus Biologicals	#NBP1-32822	AB_10003994	1 : 1000
TBP	Cell Signaling	#8515	AB_10949159	1 : 1000
NF-κB p65	Cell Signaling	#8242	AB_10859369	1 : 1000
P-IκBα	Cell Signaling	#2859	AB_561111	1 : 1000
IκBα	Cell Signaling	#4814	AB_390781	1 : 1000
P-ERK	Cell Signaling	#4377	AB_331775	1 : 1000
ERK	Cell Signaling	#9102	AB_330744	1 : 1000
P-p38	Cell Signaling	#9211	AB_331641	1 : 1000
p38	Cell Signaling	#9212	AB_330713	1 : 1000
P-JNK	Cell Signaling	#9251	AB_331659	1 : 1000
JNK	Cell Signaling	#9252	AB_2250373	1 : 1000
P-c-Jun	Cell Signaling	#3270	AB_2129575	1 : 1000
c-Jun	Cell Signaling	#9165	AB_2130165	1 : 1000
c-Fos	Cell Signaling	#2250	AB_2247211	1 : 1000
P-JAK2	Cell Signaling	3771	AB_330403	1 : 1000
JAK2	Cell Signaling	3230	AB_2128522	1 : 1000
P-STAT1	Cell Signaling	9167	AB_561284	1 : 1000
STAT1	Cell Signaling	14994	AB_2737027	1 : 1000
P-STAT3	Cell Signaling	9145	AB_2491009	1 : 1000
STAT3	Cell Signaling	12640	AB_2629499	1 : 1000
2nd anti-mouse	Cell Signaling	#7076	AB_330924	1 : 5000
2nd anti-rabbit	Cell Signaling	#7074	AB_2099233	1 : 5000