lncRNA-SNHG14 Plays a Role in Acute Lung Injury Induced by Lipopolysaccharide through Regulating Autophagy via miR-223-3p/Foxo3a

Figure 1

lncRNA-SNHG14/miR-223-3p/Foxo3a expression level altered in LPS-treated A549 cells and lung tissue collected from LPS-induced ALI models. (a) RNA-sequence analysis was used to reveal the microRNAs (left), mRNAs (middle), and lncRNAs (right) profiles in A549 cells treated by LPS for 24 h. Differentially expressed genes were shown in the volcano plot. According to the adjusted value, the top ten differentials expressed microRNAs (left), mRNAs (middle), and lncRNAs (right) were shown in heat maps. A549 cells were treated by LPS for 24 h. miR-223-3p (c), Foxo3a mRNA (d), Foxo3a protein (e), and lncRNA-SNHG14 (f) were detected using qRT-PCR and western blot, respectively. Lung tissue samples were collected from LPS-induced ALI models. miR-223-3p (g), Foxo3a mRNA (h), Foxo3a protein (i), and lncRNA-SNHG14 (j) were measured. compared with the control or Sham group.

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