lncRNA-SNHG14 Plays a Role in Acute Lung Injury Induced by Lipopolysaccharide through Regulating Autophagy via miR-223-3p/Foxo3a

Figure 2

lncRNA-SNHG14 regulated expression of miR-223-3p/Foxo3a in A549 cells. pcDNA3.1-SNHG14 and ASO-SNHG14 were transfected into A549 cells. After 48 h, miR-223-3p (a), Foxo3a mRNA (b), and Foxo3a protein (c) by qRT-PCR and western blot. miR-223-3p was transfected or cotransfected with pcDNA3.1-SNHG14, Foxo3a mRNA (d), and Foxo3a protein (e) were detected by qRT-PCR and western blot. ASO-SNHG14 was transfected into cells 24 h ahead of LPS treatment. Under LPS treating condition, miR-223-3p (f), Foxo3a mRNA (g), and Foxo3a protein (h) were detected. compared with transfection negative control or LPS untreated control. # compared with miR-223-3p mimic+pcDNA3.1 or LPS + ASO-NC.

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