lncRNA-SNHG14 Plays a Role in Acute Lung Injury Induced by Lipopolysaccharide through Regulating Autophagy via miR-223-3p/Foxo3a

Figure 6

lncRNA-SNHG14/miR-223-3p/Foxo3a axis regulated autophagy in A549 cells. (a) A549 cells were transfected with pcDNA3.1-SNHG14, miR-223-3p inhibitor, pcDNA3.1-SNHG14 + siRNA-Foxo3a, and miR-223-3p inhibitor+siRNA-Foxo3a. Autophagy markers including LC3B II/I and p62 were detected using western blotting. (b) Cells were transfected with ASO-SNHG14, ASO-SNHG14 + pcDNA3.1-Foxo3a, miR-223-3p mimic, and miR-223-3p mimic+pcDNA3.1-Foxo3a, respectively. Autophagy markers were detected under LPS treating condition. (c) Cells were treated with rapamycin or 3-methyladenine. Cell viability (c), apoptosis (d), and cytokines (e) were measured under LPS treating condition. compared with transfection negative control or LPS untreated control; ^# compared with cells without cotransfection of siRNA-Foxo3 or pcDNA3.1-Foxo3a in (a, b). ^# compared with cell without treatment of rapamycin and 3-methyladenine in (c, e).

(a)

(b)

(c)

(d)

(e)