Antituberculosis Drugs (Rifampicin and Isoniazid) Induce Liver Injury by Regulating NLRP3 Inflammasomes

Figure 5

INH and RIF regulated drug-metabolizing enzymes and induced an inflammatory response and OS in hepatic cells by activating the NLRP3 inflammasome. Hepatic cells were treated with INH and RIF and then transfected with NLRP3 siRNA. (a) Transfection efficiency of siRNAs was detected by using QRT-PCR. (b) QRT-PCR analyses were performed to detect the levels of NAT2 and CYP2E1 expression in each group of hepatocytes. ELISA assays were performed to detect the levels of IL-33 (c), IL-18 (d), and IL-1β (e) in hepatic cells treated with INH and RIF, and INF39 or CP-456773. (f) Western blot analyses revealed changes in the levels of NLRP3, ASC. And cleaved-caspase 1 protein expression in the treated hepatic cells. (g) Protein expression was quantified based on the gray values obtained from western blotting. (h) IF assays were performed to determine the expression and distribution of NLRP3 protein in treated hepatic cells. Magnification, ×200; . (i) ROS levels were monitored by flow cytometry. vs. the normal group; ^# and ^## vs. the INH and RIF group.

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