Early Reciprocal Effects in a Murine Model of Traumatic Brain Injury and Femoral Fracture

Figure 2

Femoral fracture increases structural brain damage after TBI. (a) Representative images of cresyl violet-stained brain sections, bregma -2.00 mm. Scale bar: 1 mm. (b) Lesion volume expressed as percentage of the ipsilesional hemisphere was not significantly altered in CCI versus CCI+FF mice. (c) Representative images of ipsi- and contralateral dentate gyrus of sham, CCI, and CCI+FF mice, bregma -2.00 mm, showing substance loss in the granular cell layer (GCL). (d) GCL width was determined in the suprapyramidal blade and calculated as ipsi- to contralateral ratio. CCI and CCI+FF showed reduced ratios compared to sham and FF, indicating a posttraumatic substance loss of ipsilesional GCL after CCI. CCI+FF showed significant reduction of GCL width compared to isolated CCI. Differences of CCI or CCI+FF compared to FF or sham, ^#differences between CCI and CCI+FF. Values represent ; values were calculated by Student’s -test (b) and Holm-Sidak’s multiple comparison test (d).

(a)

(b)

(c)

(d)