Early Reciprocal Effects in a Murine Model of Traumatic Brain Injury and Femoral Fracture

Figure 5

Concomitant TBI alters gene expression in the fractured femur. Representative X-ray images of the osteosynthetic stabilized femoral fracture during (a) surgical procedure and (b) 5 dpi post mortem, fracture gaps are enlarged for detailed view. Scale bar: 1 cm. (c–j) Gene expression analyses in the perifractural bone tissue normalized to GAPDH and to sham 5 dpi (/group). Gene expression of Runx2 (Runx2, c), bone sialoprotein (Ibsp, d), alkaline phosphatase protein (Alpl, e), and osteocalcin (Bglap, f) was increased in fractured bones of the FF group compared to uninjured femora of the isolated CCI group, but significantly reduced by the additional CCI in CCI+FF mice. Gene expression of osteopontin (Spp1, g) was increased in CCI+FF mice compared to sham. Gene expression of BDNF (Bdnf, h) was increased in CCI+FF compared to isolated CCI but the differences failed statistical significance (). No significant differences were revealed for the gene expressions of MRC1 (Mrc1, i) and progranulin (Grn, j). Values represent ; values were calculated by Holm-Sidak’s multiple comparison test.

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