Long-Term Follow-Up, Association between CARD15/NOD2 Polymorphisms, and Clinical Disease Behavior in Crohn’s Disease Surgical Patients

Table 2

Primers and PCR condition used for the detection of R702W, G881R, and 3020insC polymorphisms in NOD2 gene.

Exon	Polymorphism	Primers	Annealing temperature
4	Arg702Trp (R702W)	5-AGATCACAGCAGGCCTTCCTG-3; 5-GCCAATGTCACCCACAGAGT-3	59°C
8	Gly881Arg (G881R)	5-AAGTCTGTAATGTAAAGCCAC-3; 5-CCCAGCTCCTCCCTCTTC-3	55°C
11	3020insC	5-CTCACCATTGTATCTTCTTTTC-3; 5-GAATGTCAGAATCAGAAGGG-3	55°C