Topical Administration of 15-Deoxy-<i>Δ</i><sup>12,14</sup>-Prostaglandin J<sub>2</sub> Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice

<div>Schematic protocol approaching the treatment with topical formulation containing 15d-PGJ<sub>2</sub> (TFcPGJ<sub>2</sub>), time points of sample collection, and parameters analyzed at each time point. Mice were treated with TFcPGJ<sub>2</sub> or unloaded topical formulation (uTF) 1 h and 5 min before the beginning of irradiation and 5 min after the irradiation. Dorsal skin samples were collected 12 h after the exposure to UVB irradiation for skin edema, MPO and MMP-9 activity, FRAP, ABTS, total sulfhydryl, GSH levels, and histology. Samples were collected after 2 hours of exposure to UVB for the tests of catalase and superoxide anion production (NBT reduction). Finally, samples collected 4 hours after the end of the irradiation were tested for cytokines and lipid peroxidation (LPO) and mRNA expression by RT-qPCR.</div>

Mediators of Inflammation

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Figure 1

Figure 1: Topical Administration of 15-Deoxy-<i>Δ</i><sup>12,14</sup>-Prostaglandin J<sub>2</sub> Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice 

Figure 1 | Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice 