Topical Administration of 15-Deoxy-Δ^12,14-Prostaglandin J₂ Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice

Figure 4

Topical formulation containing 15d-PGJ₂ (TFcPGJ₂) inhibits the depletion of endogenous catalase activity and the enhanced production of lipid peroxides and superoxide anion as well as reduces gp91^phox and cyclooxygenase (COX-2) mRNA expression in UVB-irradiated skin. Catalase activity (a) was determined in samples collected 2 h after the end of irradiation. Lipid peroxidation (b) was measured by a chemiluminescence (QL) assay initiated by tert-butyl hydroperoxide (LOOH) on samples collected 4 h after the end of irradiation. Superoxide anion production (c) was measured by the nitroblue tetrazolium (NBT) reduction test in samples collected 2 h after the end of irradiation. NADPH oxidase subunits gp91^phox (d) and COX-2 (e) were determined in samples collected 4 h after the end of irradiation by RT-qPCR. NC: negative control; PC: positive control; uTF: unloaded topical formulation. Bars are of 6 mice per group and are representative of two separate experiments. One-way ANOVA followed by Tukey’s test. versus the negative control group; ^# versus UVB-stimulated groups.

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