17β-Estradiol Attenuates LPS-Induced Macrophage Inflammation In Vitro and Sepsis-Induced Vascular Inflammation In Vivo by Upregulating miR-29a-5p Expression

Figure 2

LPS induces the expression of NLRP3 and IL-1β in cultured RAW 264.7 cells. (a, b) RAW 264.7 cells were treated with the indicated concentrations of LPS for 24 h. qRT-PCR (a) and Western blotting (b) detected the mRNA and protein expression of IL-1β and NLRP3. Relative expression of IL-1β and NLRP3 mRNA was presented after normalizing to GAPDH (; ). vs. the LPS 0 μg/ml group. (c, d) RAW 264.7 cells were treated with 1 μg/ml LPS for the indicated times, and the mRNA and protein expression of IL-1β and NLRP3 was detected by qRT-PCR (c) and Western blotting (d). Relative expression of IL-1β and NLRP3 mRNA was presented after normalizing to GAPDH (; ). and vs. the 0 h group, respectively.

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