17β-Estradiol Attenuates LPS-Induced Macrophage Inflammation In Vitro and Sepsis-Induced Vascular Inflammation In Vivo by Upregulating miR-29a-5p Expression

Figure 5

miR-29a-5p reduces inflammation response in RAW 264.7 cells induced by LPS. (a) RAW 264.7 cells were transfected with the miR-29a-5p mimic or mimic-ctl with or without LPS (1 μg/ml) for 24 h. qRT-PCR was applied to detect mRNA expression of IL-1β and NLRP3. Relative expression of IL-1β and NLRP3 mRNA was presented after normalizing to GAPDH (; ). vs. the mimic-ctl group. ^### vs. the mimic-ctl+LPS group. (b) Western blotting detected IL-1β and NLRP3 expression in RAW 264.7 cells treated in (a). Left panel: representative blot from three independent experiments. Right panel: quantitation of IL-1β and NLRP3 normalized to β-actin. vs. the mimic-ctl group. ^### vs. the mimic-ctl+LPS group.

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