Inverse Agonist of Retinoid-Related Orphan Receptor-Alpha Prevents Apoptosis and Degeneration in Nucleus Pulposus Cells via Upregulation of YAP

Figure 4

Effect of SR3335 on NP cell viability and apoptosis. (a) NP cells were treated with the indicated concentration of SR3335 (0.5, 1.0, and 5.0 μM), and the cell viability was measured with the CCK-8 assay. . (b) The NP cells were pretreated with 1 μM of SR3335 2 h before 10 ng/ml TNF-α treatment. The protein level of cleaved caspase 3, caspase 3, BCL2, and BAX was measured by the western blot. (c) Quantification of BCL2 and BAX. The ratio of BCL2/BAX was measured. compared with the TNF-α group. (d) Quantification of cleaved caspase 3 and caspase 3. The ratio of cleaved caspase 3/caspase 3 was measured. or compared with the TNF-α group. Data are expressed as . All experiments were repeated three times.

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