Inverse Agonist of Retinoid-Related Orphan Receptor-Alpha Prevents Apoptosis and Degeneration in Nucleus Pulposus Cells via Upregulation of YAP

<div>The anticatabolism and proanabolism effect of SR3335 is mediated by interaction with ROR<i>α</i>. (a) The expression of ROR<i>α</i> protein after being transfected with RORA overexpression or sh-RORA lentivirus was measured with western blot. (b) The NP cells were transfected with sh-RORA lentivirus and 1 <i>μ</i>M of SR3335. The protein level of COL2A1, MMP13, and ADAMTS4 was assayed by western blot and quantification. (c) The NP cells were transfected with RORA overexpression lentivirus and 1 <i>μ</i>M of SR3335. The protein level of COL2A1, MMP13, and ADAMTS4 was assayed by western blot and quantification. All experiments were repeated three times.</div>

Mediators of Inflammation

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Figure 6

Figure 6: Inverse Agonist of Retinoid-Related Orphan Receptor-Alpha Prevents Apoptosis and Degeneration in Nucleus Pulposus Cells via Upregulation of YAP 

Figure 6 | Inverse Agonist of Retinoid-Related Orphan Receptor-Alpha Prevents Apoptosis and Degeneration in Nucleus Pulposus Cells via Upregulation of YAP 