Inverse Agonist of Retinoid-Related Orphan Receptor-Alpha Prevents Apoptosis and Degeneration in Nucleus Pulposus Cells via Upregulation of YAP

Figure 7

RORα inhibition suppressed the phosphorylation and inactivation of YAP induced by TNF-α. (a) The expression levels of YAP and p-YAP were measured by western blot and quantification of p-YAP and YAP. The ratio of p-YAP/YAP was calculated. Data are expressed as . and compared with the TNF-α-treated group. (b) Immunofluorescence staining of YAP showed the location and relative fluorescence strength under vehicle, TNF-α, TNF-α+SR3335 stimulation. . (c) NP cells were treated with various dosages of SR3335 (0, 0.5, 1.0, and 5.0 μM) for 2 hours. The levels of p-YAP and YAP were assayed by western blot. (d) NP cells were treated with 10 ng/ml TNF-α, 1 μM SR3335, or 1 μM VP for 24 h. The protein level of COL2A1, MMP13, and ADAMTS4 was assayed by western blot and quantification. All experiments were repeated three times.

(a)

(b)

(c)

(d)