Inverse Agonist of Retinoid-Related Orphan Receptor-Alpha Prevents Apoptosis and Degeneration in Nucleus Pulposus Cells via Upregulation of YAP

Table 1

Primers used for real-time quantitative PCR.

Gene	Primer	Sequence (5-3)
GAPDH	Forward	AGAAAAACCTGCCAAATATGATGAC
	Reverse	TGGGTGTCGCTGTTGAAGTC
COL2A1	Forward	GGCAATAGCAGGTTCACGTACA
	Reverse	CGATAACAGTCTTGCCCCACTT
ACAN	Forward	TGCATTCCACGAAGCTAACCTT
	Reverse	GACGCCTCGCCTTCTTGAA
MMP13	Forward	GAGGAGGAGATCGTGTTTCCA
	Reverse	CCAGCTCTAGTAGCAGCGTC
ADAMTS4	Forward	CCAGACTTCACGATGGCATTG
	Reverse	GGCATCTCCTCCATAATTTGGC

GAPDH: glyceraldehyde-3-phosphate dehydrogenase; COL2A1: collagen type II; ACAN: aggrecan; MMP13: matrix metalloproteinase 13; ADAMTS4: a disintegrin and metalloproteinase with thrombospondin motifs 4.