NRF2 Exerts Anti-Inflammatory Effects in LPS-Induced gEECs by Inhibiting the Activation of the NF-κB

Figure 3

Depleting NRF2 increased LPS-induced proinflammatory cytokines expression. Control and NRF2 knockout (KO) gEECs were stimulated with or without 8 μg/mL LPS for 12 hours and then (a) detected the NRF2 protein expression by immunoblotting. (b) The dynamic changes of cytoplasmic and nuclear NRF2 were imaged by immunofluorensent staining. (c) RT-qPCR analysis of the indicated TNF-α, IL-1β, IL-6, and IL-8 mRNA expression. (d) The concentrations of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8) in culture medium were determined with ELISA. Data shown are ; ns was P > 0.05, and ### was vs. control group; was vs. WT group. value was calculated by the one-way ANOVA with Tukey’s test as a posthoc test. Scale bar was 45 μm.

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