NRF2 Exerts Anti-Inflammatory Effects in LPS-Induced gEECs by Inhibiting the Activation of the NF-κB

Figure 4

Depleting NRF2 increased LPS-induced the activation of TLR4/NF-κB pathway. Control and NRF2 knockout (KO) gEECs were stimulated with or without 8 μg/mL LPS for 12 hours and (a) then detected protein expression by immunoblotting and quantified TLR4 (b), p-IκBα/IκBα (c), and p-p65/p65 (d) protein expression. Data shown are ; ns was , and # was vs. control group; was vs. WT group. value was calculated by the one-way ANOVA with Tukey’s test as a posthoc test.

(a)

(b)

(c)

(d)