NRF2 Exerts Anti-Inflammatory Effects in LPS-Induced gEECs by Inhibiting the Activation of the NF-κB

Figure 5

NRF2 binds to p65 in the nucleus, hindering the DNA binding activity of p65. GEECs were harvested and prepared cell lysis after treating with 8 μg/mL LPS for 12 hours. (a) Immunoprecipitation with NRF2 antibody (Rabbit IgG was used as negative control) and immunoblotting with NRF2, TLR4, IκBα, and p65 antiboty. (b) Prokaryotic expression of NRF2 protein with his label was incubated with protein A/G beads coprecipitated with NRF2 antibody for 12 hours and immunoblotting with NRF2 and his antibody. (c) Immunoblotting with NRF2 antibody, and (d) ChIP assays were performed with IgG and anti-p65 antibody; ChIP values are shown as fold changes of immunoprecipitated promoter fragments over IgG controls. was vs. control group, and value was calculated by Student’s -test.

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