NRF2 Exerts Anti-Inflammatory Effects in LPS-Induced gEECs by Inhibiting the Activation of the NF-κB

Figure 7

Depleting proinflammatory cytokine expression decreased LPS-induced NRF2 expression and nuclear abundance of NRF2. 72 hours after transferring si-RNA to gEECs, (a) RT-qPCR was used to detect the expression of TNF-α, IL-1β, IL-6, and IL-8 mRNA. (b) Immunoblotting was used to detected the expression of NRF2. (c) The dynamic changes of cytoplasmic and nuclear NRF2 were imaged by immunofluorensent staining. was vs. si-NC group, and value was calculated by the one-way ANOVA with Tukey’s test as a posthoc test. Scale bar was 45 μm.

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