|
| Primers used in RT-qPCR analysis |
|
| TNF-α | Forward: 5-ATAACAAGCCGGTAGCCCAC-3 |
| Reverse: 5-CTCAGTCTGGATTCAGCCCC-3 |
| IL-1β | Forward: 5-CGCATGTTCCTGGGGAGATT-3 |
| Reverse: 5-TGGGATGCAACATGGCTCTT-3 |
| IL-6 | Forward: 5-GCTTCTGCATTGGGAGGTCT-3 |
| Reverse: 5-ACAGGACATAGTCTGCCCCT-3 |
| IL-8 | Forward: 5-CATGCCTGGATAGCAAGCCT-3 |
| Reverse: 5-TGATAGCTGCCTGAAGCTCG-3 |
| β-Actin | Forward: 5-GGCTGTATTCCCCTCCATCG-3 |
| Reverse: 5-CCAGTTGGTAACAATGCCATGT-3 |
|
| Primers used in ChiP analysis |
| TNF-α | Forward: 5-GAGGCAATAGGTTTTGAGG-3 |
| Reverse: 5-AAGCATCAAGGATACCCTC-3 |
| IL-1β | Forward: 5-ACCCTCACCCTCCAACAAAG-3 |
| Reverse: 5-TGGAAGGGCAAGGAGTAGCA-3 |
| IL-6 | Forward: 5-GTGTCTTCCACTTTGTCCCACA-3 |
| Reverse: 5-TGGAAGGGCAAGGAGTAGCA-3 |
| IL-8 | Forward: 5-GGCTTCCCTGATAGCTCAGTT-3 |
| Reverse: 5-TGAACCCAGGTCTACCCACAT-3 |
|
| si-RNA sequence |
| si-NC | Forward: 5-AAGUUCAGGACUAAGUCAGGC-3 |
| Reverse: 5-GAGACUAUGACUCGUAAUUAC-3 |
| si-TNF-α | Forward: 5-UCUUUCUCUCUCAUUUCUCUC-3 |
| Reverse: 5-GAGAAAUGAGAGAGAAAGAGG-3 |
| si-IL-1β | Forward: 5-ACUGUAAUGAAAACAGAUGUG-3 |
| Reverse: 5-CAUCUGUUUUCAUUACAGUGA-3 |
| si-IL-6 | Forward: 5-AAGGAUUUCCUUCACUUACUU-3 |
| Reverse: 5-GUAAGUGAAGGAAAUCCUUAG-3 |
| si-IL-8 | Forward: 5-AUUAGAAGAAUAUGCUUACCU-3 |
| Reverse: 5-GUAAGCAUAUUCUUCUAAUCU-3 |
|
