The Modulation of Interferon Regulatory Factor-1 via Caspase-1-Mediated Alveolar Macrophage Pyroptosis in Ventilator-Induced Lung Injury

Figure 1

Ventilation with a high tidal volume induces elevated caspase-1-dependent pyroptosis in AMs. Caspase-1-related death cells detected by flow cytometry (a) and the protein level of GSDMD including full-length and cleaved forms (b) and mature IL-1β (c) in alveolar macrophages were increased in high VT group compared with the group of control and low VT. The release of IL-1β in BALF (d) and serum (e) was significantly increased after ventilation with high VT than that in the low VT group, compared with control group. Results are representative of three independent experiments; the results of one representative experiment are shown (/group, , ).

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