The Modulation of Interferon Regulatory Factor-1 via Caspase-1-Mediated Alveolar Macrophage Pyroptosis in Ventilator-Induced Lung Injury

Figure 4

IRF-1 deletion attenuates VILI and cytokine release in mice. The intranuclear protein level (a) and mRNA (b) of IRF-1 in alveolar macrophages were increased in the high VT group compared with the control group and the low VT group. IRF-1 deletion alleviated lung histopathologic damage in gross pathology (e), HE-stained micrographs for 20× magnification (f) and 400× magnification (g) induced by the high-tidal-volume ventilation assessed using lung injury scores (h). IRF-1 deletion alleviated the wet/dry (W/D) ratio (c) and BALF protein concentration (d) in the high VT group. The concentration of IL-6 (i) and TNF-α (j) in BALF was attenuated after high VT with IRF-1 deletion compared to that wild type. Results are representative of three independent experiments; the results of one representative experiment are shown (/group, , ).

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