The Modulation of Interferon Regulatory Factor-1 via Caspase-1-Mediated Alveolar Macrophage Pyroptosis in Ventilator-Induced Lung Injury

Figure 5

IRF-1 was required for caspase-1 activation in AMs. The flow cytometry showed IRF-1 deletion attenuated alveolar macrophage pyroptosis in high VT (a). Western blotting analysis of protein expression of caspase-1 p10 (b), IL-1β (b), and GSDMD including full-length and cleaved forms (c) in alveolar macrophages. Analysis of IRF-1 protein levels (d) in the nucleus of alveolar macrophages indicated that caspase-1 deletion did not affect expression of IRF-1. The concentration of IL-1β (e) and HMGB-1 (f) in BALF was attenuated after high VT with IRF-1 deletion compared to that wild type. Results are representative of three independent experiments; the results of one representative experiment are shown (/group, , ).

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