Cayratia japonica Prevents Ulcerative Colitis by Promoting M2 Macrophage Polarization through Blocking the TLR4/MAPK/NF-κB Pathway

Figure 4

CJ inhibits the macrophage M1 polarization and enhances the macrophage M2 polarization. (a) NO concentration measured by the Griess reagent. (b) IL-6, IL-1β, and TNF-α levels in the cells detected by ELISA. (c) mRNA expression of IL-6, IL-1β, and TNF-α levels detected by RT-qPCR. (d) The protein expression of M1 macrophage markers and M2 macrophage markers in the cells measured by Western blot assay. (e) Determination of CD11b⁺ (M1 type macrophages) and CD206⁺ (M2 type macrophages) signals in RAW264.7 cells. ; ; ; . Cell experiments were repeated three times.

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