Cayratia japonica Prevents Ulcerative Colitis by Promoting M2 Macrophage Polarization through Blocking the TLR4/MAPK/NF-κB Pathway

Figure 5

CJ affects macrophage polarization and immune activity via the TLR4/MAPK/NF-κB pathway. (a) mRNA levels of TLR4 measured by RT-qPCR. LPS (1 μg/mL); CJ (200 μg/mL). (b) TLR4 protein expression in macrophages by ELISA. (c) Total and phosphorylated protein levels of p38, ERK, JNK, and NF-κB p65 in macrophages detected by ELISA. (d) The protein expression of CD206 and CD11b in macrophages measured by Western blot assay. (e) IL-6, IL-1β, and TNF-α levels in macrophages detected by ELISA. (f) Intracellular NO concentration determined by the Griess reagent. (g) Determination of CD11b⁺ (M1 type macrophages) and CD206⁺ (M2 type macrophages) signals in RAW264.7 cells. ; ; ; . Cell experiments were repeated three times.

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