Vaspin Alleviates Sepsis-Induced Cardiac Injury and Cardiac Inflammation by Inhibiting Kallikrein 7 in Mice

Figure 7

Effects of KLK7 deletion on cardiac inflammation and cardiomyocyte apoptosis in septic mice. (a, b) Levels of CD45+ cells and CD68+ cells, as well as the levels of the proinflammatory cytokines MCP-1, IL-6, IL-17, TNF-α, and IFN-γ in the heart (two-way ANOVA). Blue, green, and red indicate nuclei, CD45-positive cells, and CD68-positive cells, respectively. (c) The expression of cleaved caspase3 in LPS-treated WT cardiomyocytes and KLK7-/- cardiomyocytes was analyzed (two-way ANOVA). Blue and red spots indicate the nucleus and cleaved caspase3, respectively. (d) The number of TUNEL-positive cardiac cells was quantified (two-way ANOVA). vs. the or group. ^ns vs. the or group.

(a)

(b)

(c)

(d)