Network Pharmacology Analysis and Experimental Validation to Explore the Anti-inflammatory Mechanism of Asiatic Acid on Alcoholic Steatohepatitis

Figure 8

Asiatic acid may inhibit alcohol-induced inflammation by regulating NF-κB pathway in the RAW264.7 cells. (a) Relative cell viability of RAW264.7 cells. (b) Relative mRNA expression of IL-1β, IL-6, and TNF-α in the RAW264.7 cells. (c–e) Levels of f IL-1β, IL-6, and TNF-α in the supernatant of RAW 264.7 cells. (f) Relative protein expression of IL-1β, IL-6, and TNF-α in the RAW264.7 cells. (g) Relative protein expression of P-NF-κB-p65 in the RAW264.7 cells. (h) Representative immunofluorescence images of P-NF-κB-p65 (red) (×200) in the control and EtOH groups are presented (). compared with the Control group. compared with the EtOH group. The data represent the of at least three independent experiments.

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