Research Article
Knockdown of LMX1B Suppressed Cell Apoptosis and Inflammatory Response in IL-1β-Induced Human Osteoarthritis Chondrocytes through NF-κB and NLRP3 Signal Pathway
Figure 2
LMX1B knockdown increased IL-1β-induced SW1353 cell growth and suppressed its apoptosis. (a) Western blot analysis for LMX1B protein expression; (b) CCK-8 analysis for SW1353 cell survival; (c) 5-ethynyl-2-deoxyuridine (EdU) assay was used to determine SW1353 cell proliferation; (d) SW1353 cell apoptosis was detected by using flow cytometer analysis. SW1353 cells were transfected with control siRNA or LMX1B siRNA for 24 h by using Lipofectamine 2000 reagent and then treated with 10 ng/ml IL-1β for 24 h. , compared with the control group; #, compared with the IL-1β group. All experiments were repeated three times.
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