Research Article
Treatment with Commelina communis Extract Exerts Anti-inflammatory Effects in Murine Macrophages via Modulation of the Nuclear Factor-κB Pathway
Figure 4
Effect of Commelina communis extract (CCE) treatment on lipopolysaccharide- (LPS-) induced production of cytokines such as tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1β, and IL-6 in RAW 264.7 cells; protein expression (a–c) and mRNA expression (d–f). Cells were pretreated with the indicated concentrations of CCE (0.5, 1, and 1.5 mg/mL) for 4 h and then exposed to LPS for 24 h. Cell supernatants were analyzed using enzyme-linked immunosorbent assay and real-time polymerase chain reaction. The experimental results, in triplicate, are indicated as the . # compared with the control; compared with the LPS-exposed group.
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