Treatment with Commelina communis Extract Exerts Anti-inflammatory Effects in Murine Macrophages via Modulation of the Nuclear Factor-κB Pathway

Figure 5

Commelina communis extract (CCE) treatment hinders activation of the p65 subunit of nuclear factor- (NF-) κB in RAW 264.7 cells. Murine macrophages were treated with CCE for 4 h and then exposed to lipopolysaccharide (LPS) for 1 h. (a) NF-κB p65 expression levels in the nuclear and cytosolic protein fractions were detected using western blotting and quantified using standard solutions of PCNA (b) and β-actin (c), respectively. (d) Nuclear NF-κB was assessed using enzyme-linked immunosorbent assay. Data are expressed as the from three independent experiments. ^# compared with the control; compared with the LPS-exposed group.

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