Treatment with Commelina communis Extract Exerts Anti-inflammatory Effects in Murine Macrophages via Modulation of the Nuclear Factor-κB Pathway

Table 1

Primer sequences used for real-time polymerase chain reaction and conditions for detection of proinflammatory gene expression.

Genes	Sequence (5-3)	Size (bp)	Tm (°C)	Cycles
TNF-α	Forward: 5-GGCAGGTCTACTTTGGAGTCATTGC-3; Reverse: 5-ACATTCGAGGCTCCAGTGAATTCGG-3	305	65	40
IL-1β	Forward: 5-GAAAGACGGCACACCCACCCT-3; Reverse: 5-GCTCTGCTTGTGAGGTGCTGATGTA-3	167	65	40
IL-6	Forward: 5-TCAGAATTGCCATTGCACA-3; Reverse: 5-GTCGGAGGCTTAATTACACATG-3	74	55	45
iNOS	Forward: 5-AAGTCAAATCCTACCAAAGTGA-3; Reverse: 5-CCATAATACTGGTTGATGAACT-3	409	65	40
β-Actin	Forward: 5-CATCACTATCGGCAATGAGC-3; Reverse: 5-GACAGCACTGTGTTGGCATA-3	159	65	40