Continuous Theta-Burst Stimulation Promotes Paravascular CSF-Interstitial Fluid Exchange through Regulation of Aquaporin-4 Polarization in APP/PS1 Mice

Figure 1

Study protocol. (a) Experimental timeline. Timeline showing a time series event of mouse treatment. APP/PS1 mice were first subjected to continuous theta-burst stimulation (CTBS; Real, AR; Sham, AS) for four weeks, and then the spatial cognition function was tested in the Morris water maze (MWM). Two-photon imaging was used to detect the function of the glymphatic system within two days after the MWM was completed, and the sample tissue was collected within 2 hours after the imaging was completed. (b) A schematic diagram of the mouse cranial window. A thinned cranial window was prepared and fixed on a metal plate. (c). Schematic diagram of Cisterna magna injection. After the cranial window was prepared, rhodamine was injected into the tail vein to label the blood vessels, and then FITC was injected through cisterna magna to prepare for imaging.

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