Targeting NLRP3 Inflammasome Alleviates Synovitis by Reducing Pyroptosis in Rats with Experimental Temporomandibular Joint Osteoarthritis

Figure 5

LPS+ATP stimulated NLRP3 inflammasome- and pyroptosis-related molecules in the cultured FLS cells alone and cocultured system. (a, b) When FLS cells were cultured alone, lysate was collected for western blot after being treated with LPS+ATP. (c) Total RNAs of FLS cells were extracted for detecting mRNAs of NLRP3, ASC, caspase-1, GSDMD, and IL-1β. Statistical analysis is run by independent samples -test. (d, e) Secretion of IL-1β and IL-18 in the supernate increased when FLS cell was cocultured with active THP-1 cell. (f–h) Western blotting and qRT-PCR results of cell lysate indicated the growing expression of NLRP3, ASC, caspase-1, GSDMD, and IL-1β on protein and mRNA levels. Both the upregulation trend of the above tests after LPS+ATP intervention could be inhibited by MCC950 and AYC in the coculture system. Data are presented as ; one-way ANOVA followed by Bonferroni’s post hoc test was adopted for analysis. , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)