Phosphorylation at Ser 727 Increases STAT3 Interaction with PKCε Regulating Neuron–Glia Crosstalk via IL-6-Mediated Hyperalgesia In Vivo and In Vitro

Figure 5

Expression of proteins related to activated neurocytes detected by Western blot analysis. (a–i) Protein expression of pSTAT3 (Ser727), IL-6, c-Fos, GFAP, and Iba-1 in the spinal cords of FCA-treated rats significant decreased () after intrathecal injections of PKCε inhibitor peptide (100 μg/50 μL), APTSTAT3-9R (20 μg/50 μL), and anti-IL-6 antibody (100 ng/50 μL). Values were normalized against GAPDH and are expressed as ratios (%) of control values. Data are shown as (–5). , , ; one-way ANOVA followed by Bonferroni tests.

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