Rapamycin Alleviates 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis through Autophagy Induction and NF-κB Pathway Inhibition in Mice

Figure 3

Rapamycin induces autophagy in vitro. HT-29 cells were stimulated by LPS (100 ng/ml) with or without rapamycin (25 μg/ml) pretreatment for 1 h. (a) The formation of autophagic corpuscles in HT-29 cells were detected by MDC. (b) The intracellular autophagosomes were observed using electron microscopy (arrow). (c) The expression of ATG16L1, LC3I, and LC3II in HT-29 cells was detected by western blot per group; .

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