Rapamycin Alleviates 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis through Autophagy Induction and NF-κB Pathway Inhibition in Mice

Figure 4

Rapamycin inhibits p65 nucleus translocation and TNF-α secretion both in vivo and in vitro. Mice were treated as the same in Figure 1 for 7 days. (a) The expression of p65 in colon tissue was detected using IHC staining (×100). (b) The expression of p65 in colon tissue was detected using western blot. (c) The expression of TNF-α from colon tissue was detected by ELISA ( per group). (d) HT-29 cells were stimulated by LPS (100 ng/ml) with or without rapamycin (25 μg/ml) pretreatment for 1 h. The expression of p65 was detected by western blot. (e) The expression of TNF-α from HT-29 cells was detected by ELISA. per group; .

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