miR-559 Inhibits Proliferation, Autophagy, and Angiogenesis of Hepatocellular Carcinoma Cells by Targeting PARD3

Figure 5

PARD3 silencing reversed the effects of the miR-559 inhibitor on Huh-7 cells. Huh-7 cells were cotransfected with the miR-559 inhibitor and si-PARD3 for 24 h. The mRNA (a) and protein (b) levels of PARD3 in Huh-7 cells. (c) Using the CCK8 assay to detect cell viability. (d) The expression levels of LC3-II/I, Beclin1, and p62 were measured by the western blot assay. (e) HUVEC tube formation assay was proposed using conditioned medium in vitro. (f) The protein levels of VEGF and Ang-2 were detected by western blotting in Huh-7 cells. The values were obtained from three independent experiments and shown as . , , and , compared with inhibitor NC; ^## and ^###, compared with the miR-559 inhibitor.

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