Edaravone Dexborneol Downregulates Neutrophil Extracellular Trap Expression and Ameliorates Blood-Brain Barrier Permeability in Acute Ischemic Stroke

Figure 4

Protective effects of Eda.B against NETs and inflammatory factors. (a, b) The levels of GSH-Px and SOD in different groups were quantified by commercial kits. (c) mRNA expression of Tnf in mouse cortex at day 1 and day 3 after stroke.(d–g) Protein levels of TNFα and citH3 at day 1 and day 3 after stroke. Representative bands are on the top. (h) Representative images of sections stained with citH3 (red), neutrophil marker Ly6G (green), and DAPI (blue) in infarct cortex.  μm. (i, j) Representative images of sections stained with citH3 (red) and DAPI (blue) in infarct cortex and quantification of NETs in different groups.  μm. (MCAO group vs. Sham group), ^# (Eda.B group or DNase I group vs. MCAO group), and ^## (Eda.B group or DNase I group vs. MCAO group), in each group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)