Methylation Mediated Silencing of miR-155 Suppresses the Development of Preeclampsia In Vitro and In Vivo by Targeting FOXO3

Figure 2

miR-155 regulated cell viability, apoptosis, mobility, and oxidative stress in trophoblast cells. Negative control (NC), miR-155 mimic, and the miR-155 inhibitor were transfected into HTR8/SVneo and JEG-3 cells. (a) RT-qPCR assay was used to measure the expression of miR-155 in the cells. (b, c) Cell Counting Kit-8 (CCK-8) assay was used to measure the cell viability after transfection in HTR8/SVneo and JEG-3 cells. (d) Detection of cell apoptosis by TUNEL assay. The nuclei of apoptotic cells had green fluorescence. Hoechst staining showed that the nuclei of placental trophoblast cells were blue-violet. Microscopic images and quantitative analysis of Transwell migration (e) and invasion (f) in cells following transfection. (g) Western blot analysis was performed to determine the expression levels of proteins associated with apoptosis (CytC), invasion (MMP2), and oxidative stress (SOD1). (h) RT-qPCR assay and (i) ELISA were used to measure the expression of MMP2 in the cells. , , and .

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