Methylation Mediated Silencing of miR-155 Suppresses the Development of Preeclampsia In Vitro and In Vivo by Targeting FOXO3

Figure 3

5-Aza treatment attenuated the effects of miR-155 knockdown on trophoblast dysfunction in vitro. (a) RT-qPCR analysis of miR-155 indicated that the miR-155 inhibitor-evoked downregulation of miR-155 was reversed by 5-Aza treatment of HTR8/SVneo and JEG-3 cells. (b) 5-Aza inhibited methylation level of miR-155 promoter in HTR8/SVneo and JEG-3 cells. (c) CCK-8 assay indicated that 5-Aza reversed the promotion of cell viability by the miR-155 inhibitor in HTR8/SVneo and JEG-3 cells. (d) Detection of cell apoptosis by TUNEL assay. Transwell assays indicated that 5-Aza attenuated the increased cell migration (e) and invasion (f) induced by the miR-155 inhibitor (magnification 200×) in HTR8/SVneo and JEG-3 cells. (g) The protein levels of MMP2, CytC, and SOD1 were measured in 5-Aza-treated HTR8/SVneo and JEG-3 cells with the miR-155 inhibitor. , , and .

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