Methylation Mediated Silencing of miR-155 Suppresses the Development of Preeclampsia <i>In Vitro</i> and <i>In Vivo</i> by Targeting FOXO3

<div>miR-155 knockdown inhibited inflammation, apoptosis, and oxidative stress in trophoblast cells by upregulating FOXO3. HTR8/SVneo and JEG-3 cells were transfected with NC, inhibitor, si-NC, si-FOXO3, and inhibitor + si-FOXO3, respectively. ELISA assay was used to measure the release of (a) IL-6, (b) IL-8, and (c) TNF-<i>α</i> in cell supernatants of the above-transfected cells. (d, e) Expression levels of MMP2 were determined using RT-qPCR assay (d) and ELISA assay (e) in the above-transfected cells. (f) The protein levels of FOXO3, MMP2, CytC, and SOD1 were measured in the above-transfected cells. <span class="nowrap"><svg height="9.2729pt" id="M24" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -8.6359 52.4989 9.2729" width="52.4989pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M471 153C471 170 463 194 452 212C400 220 373 229 322 255C373 281 400 290 452 298C463 316 471 339 471 357C456 366 431 371 410 370C377 329 356 310 308 279C311 336 317 364 336 413C326 432 310 451 294 459C279 451 262 432 252 413C271 364 277 336 280 279C232 310 211 329 178 370C157 371 132 367 117 357C117 340 125 316 136 298C188 290 215 281 266 255C215 229 188 220 136 212C125 194 117 171 117 153C132 144 157 139 178 140C211 181 232 200 280 231C277 174 271 146 252 97C262 78 278 59 294 51C309 59 326 78 336 97C317 146 311 174 308 231C356 200 377 181 410 140C431 139 456 143 471 153Z"></path></g><g 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Mediators of Inflammation

fig6

Figure 6

Figure 6: Methylation Mediated Silencing of miR-155 Suppresses the Development of Preeclampsia <i>In Vitro</i> and <i>In Vivo</i> by Targeting FOXO3 

Mediators of Inflammation

Methylation Mediated Silencing of miR-155 Suppresses the Development of Preeclampsia In Vitro and In Vivo by Targeting FOXO3

Figure 6