IL-21/IL-21R Regulates the Neutrophil-Mediated Pathologic Immune Response during Chlamydial Respiratory Infection

Figure 1

Pulmonary neutrophil infiltrations of IL-21R^-/- mice in response to Chlamydia muridarum (C. muridarum) lung infection. The lung single cells of wild-type (WT) and IL-21 receptor deficient (IL-21R^-/-) mice were prepared. (a, b) Wright-Giemsa staining of lung single cells was performed, and the percentages of monocytes, neutrophils, and lymphocytes in lungs of WT and IL-21R^-/- mice following C. muridarum infection were analyzed. (c–f) Flow cytometry performed with pulmonary neutrophils represented as CD45⁺ CD11b⁺ Ly-6G⁺ cells. (c) Representative flow cytometric plots, (d) frequencies, and (e) total numbers of (f) neutrophils in the lungs were shown. (g) Lung cryosections at day 3 p.i. were stained by anti-Ly-6G for neutrophil (green), and nuclei were stained with DAPI (blue), representative immunostaining captured by fluorescence microscopy (20x). Data are represented as from per group, representative one of (c–f) five independent experiments or (a, b, g) three independent experiments. Statistical significances of differences are determined by two-way ANOVA followed by Bonferroni’s multiple comparisons test. ; .

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