Treatment with Herbal Formula Extract in the hSOD1^G93A Mouse Model Attenuates Muscle and Spinal Cord Dysfunction via Anti-Inflammation

Figure 3

Herbal formula extract (HFE) reduced the expression of inflammatory proteins and oxidative stress-related proteins in the tibialis anterior (TA) and gastrocnemius (GC) of hSOD1^G93A mice. (a) Representative immunoblots of inflammatory proteins and their quantification in the TA of nontransgenic (nTg), transgenic (Tg), and HFE-Tg mice.  =3–4 per group. (b) mRNA expression level of IL18 in the TA of each group.  =4–5 mice per group. (c) Representative blot and quantification of the level of GFAP protein in the GC of nTg, Tg, and HFE + Tg mice.  =3–4 per group. (d) Levels of oxidative stress-related proteins (HO1, ferritin, transferrrin, and BAX) were reduced by HFE treatment in the TA of hSOD1^G93A mice.  =3–4 per group. (e) mRNA expression level of COX IV 1a in the TA of each group.  =4–5 mice per group. , , . nTg: nontransgenic mice, Tg: transgenic mice, Tg + HFE: HFE-treated transgenic mice.

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