Treatment with Herbal Formula Extract in the hSOD1^G93A Mouse Model Attenuates Muscle and Spinal Cord Dysfunction via Anti-Inflammation

Figure 5

Herbal formula extract (HFE) reduced neuroinflammation in the spinal cord of hSOD1^G93A mice. (a) Representative images of ventral horn of spinal cord showing diaminobenzidine staining using anti-CHAT, anti-Iba1, and anti-GFAP. HFE administration increased CHAT-positive cells and reduced Iba1- and GFAP-positive cells in the ventral horn of the spinal cord in the Tg group. Quantification of positive intensity of primary antibody.  =3–4 mice per group. Scale bars: 2 mm. (b) Representative image of SMAD2, p62, and ferritin expression detected using western blotting analysis in the nTg, Tg, and Tg-HFE groups. Tubulin was used as the loading control. Quantification of immunoblots of GFAP, SMAD2, P62, and ferritin. Data are presented as the mean ± SEM.  =3–4 mice per group. , , . nTg: nontransgenic mice, Tg: transgenic mice, Tg + HFE: HFE-treated transgenic mice.

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